Investigation of Temperature on Catalase Activity
In conclusion to this experiment, it is evident that catalase is most active at 40 degrees Celsius. There is a trend in a graph that shows the froth increases up towards 40 degrees Celsius and dips when reaching 60 degrees Celsius. It is not just a case of the Enzymes activity decreasing it becomes denatured shortly after it reaches it's optimum level of activity.
In this evaluation, the flaws and the limitations of this experiment will be outlined. The procedures in this experiment were very effective. Unlike many other experiments the control of the variables was without fault, because a machine was controlling them, i.e. the water bath. There were not many limitations of equipment, and the only way the equipment could have been improved would have been to have the experiment monitored by computer. All the sources of error in this experiment were with the non-variables. Firstly, there was no way of being sure that each potato was the exact same length or mass. A way of improving this would be using a guillotine-type device, meaning that the potatoes were at least the exact same length since they were cut at equal angles all together. Another flaw is the amount of H2O2 poured into each separate test tube. …
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