For my investigation, I plan to examine the effect temperature has on enzyme action involving the biological catalyst- diastase, and its substrate- starch. In lesson, I have conducted a preliminary experiment to give me an idea of what to expect and a chance to conduct the experiment before hand. From my preliminary work I believe that an equal amount of substrate (Starch) with an equal amount of enzyme (Diastase) should finish reacting at around 90-120 seconds. …