In the graph, it shows the rate of enzyme reaction (O2 produced in 3 minutes) against temperature change form 10℃ to 60℃. At low temperature (10℃), the reaction of enzyme is relatively slow. As temperature increase, the rate of reaction increases. Until it reaches the optimum temperature (30℃,in this case) the reaction rates start to fall as there is further increase in temperature. The reaction drops significantly between 40℃ and 50℃. At 60 ℃, the enzyme no longer works since it has been denatured by the high temperature.
The above happens because at low temperatures particles of reacting substances do not have much energy. However, when the substances are heated, the particles take in energy. This causes them to move faster and collide more often. The collisions have more energy, so more of them are successful. Therefore the rate of reaction increases until the optimum temperature is reached.
At high temperature, the enzyme is denatured by the kinetic energy and there it no longer works.
Evaluation
There are a number of sources of error in this experiment.…
