Cell Culture Methodology From Donor to Cell Lines
Cellular tissue culture has come a long way since the beginning of the 20th century. Published papers in the early 1900¹s report a theory and a procedure, although harsh and crude, to keep cells alive in media, and those cells¹ behavior in vitro would be similar cells in vivo. The advantages of these cell cultures soon came into perspective, medical research could soar from this. Certain difficulties were soon overcome and a serum free media was produced, allowing a huge repertoire of cell lines to be produced. These cell lines created a diversity, allowing scientist to look at how different species of cells could respond to different things.
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